Prospective, Randomized Clinical Evaluation of Optisol vs Organ Culture Corneal Storage Media
نویسندگان
چکیده
منابع مشابه
A randomized, double-masked clinical trial of Optisol-GS vs Chen Medium for human corneal storage.
OBJECTIVE To determine whether the clinical outcome of corneal transplantation performed with tissue stored in Chen Medium (CM; Chen Laboratories, Phoenix, Md) is superior to that of Optisol-GS (Chiron Ophthalmics, Irvine, Calif). DESIGN Randomized, double-masked trial. PARTICIPANTS AND SETTING Ninety patients undergoing corneal transplantation at a tertiary center. INTERVENTION Donor cor...
متن کاملEffects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
PURPOSE A previous report has described the use of eye bank storage of cultured human limbal epithelial cells (HLECs) to provide a reliable source of tissue for treating limbal stem cell deficiency. In the present study, conventional organ culture (OC) storage and Optisol-GS (Bausch & Lomb, Irvine, CA) storage of cultured HLECs were compared. METHODS Three-week HLEC cultures were either organ...
متن کاملEfficiency of blood culture bottles for the fungal sterility testing of corneal organ culture media.
BACKGROUND/AIM The consequences of fungal contamination of an organ cultured cornea, though exceptional, are often disastrous for the recipient. Consequently, eye banks often quarantine corneas for 10 days or more before passing them for grafting. This period, though detrimental to the endothelial cell density of the delivered cornea, is necessary to detect contamination using conventional micr...
متن کاملDonor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan
PURPOSE Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC. METHODS Morphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT-PCR and chang...
متن کاملOrgan culture storage of pre-prepared corneal donor material for Descemet's membrane endothelial keratoplasty
PURPOSE To evaluate the effect of media composition and storage method on pre-prepared Descemet's membrane endothelial keratoplasty (DMEK) grafts. METHODS 50 corneas were used. Endothelial wound healing and proliferation in different media were assessed using a standard injury model. DMEK grafts were stored using three methods: peeling with free scroll storage; partial peeling with storage on...
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ژورنال
عنوان ژورنال: Archives of Ophthalmology
سال: 2000
ISSN: 0003-9950
DOI: 10.1001/archopht.118.6.757